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TPI-Helpathon #4 was called by three researchers at the Biomedical Primate Research Center. Anne-Marie Zeeman, Raissa Timmerman and Frank Verreck have three really good in vitro modelling questions to progress their research on liver, lung and brain diseases. Watch the video to get a glimpse of what happened in the ONLINE HELPATHON HOUSE.

HELPATHON #1 (winter 2018) and #2 (spring 2019) are still impacting the practice of the researchers involved. They successfully applied for grants to progress new animal free research. Summer 2020 Helpathon #3 went hybride. And also this Helpathon accelerated processes. Below you will find brief summary's, testimonials of the speakers and the latest updates of the progres.

HELPATHON#4

EXPLORING PROMISING NEW ORGANOID MODELS TO FIGHT ALZHEIMER, MALARIA AND TUBERCULOSIS

Again we went hybrid in TPI-HELPATHON #4, November 2nd-3rd. 60 researchers, policy makers, regulators and general members of the public volunteer to help researchers Raissa, Anne-Marie and Frank of the Biomedical Primate Research Center to explore promising new organoid models to fight Alzheimer, Malaria and Tuberculosis.

A huge THANK YOU to everyone who has donated their time and ideas …
‘Back to the future’ seems to cover well for a retrospective view on Helpathon#4 and a personal word of “thank you” to all who engaged in this event of late November 2 and 3.’ ‘I would like to thank all the helpers for their contribution, energy and open mindedness.’ Frank, Raissa, Anne-Marie and Jeffrey, all researchers from the Biomedical Primate Research Center in Rijswijk, clearly appreciated the help offered by the 60 participants of TPI HELPATHON #4.

Looking for complex in vitro models
Frank, Raissa, Anne-Marie and Jeffrey asked us to help them find in vitro models with high levels of complexity to help fight: tuberculosis, malaria and Alzheimer. They were looking for human organoid models that could mimic and thereby replace using primates as a model. During the 24 hour long Helpathon they shared practices and points of view with other participants and explored upcoming possibilities for in vitro modelling of the lungs, the liver and the brain. Fifteen of the participants were present in Mister Lion’ Helpathon studio and forty five joined in the specially conceived Online Helpathon House.

It might be important to break down complex questions
After a good night's sleep, using our famous collaborative post-it session, it became clear that the level of complexity required in an in-vitro model was directly related to the complexity of the research question. Building the right level of complexity into an in vitro model was compared by Jeffrey to solving a really tough rubik's cube: each face contains characteristics that are linked in a particular way. As soon as you change something in one face it messes up what you put in the other faces. As the helpers revisited the researchers questions they also came up with other research strategies to fight the respective diseases. Both integrating these research efforts and building the invitro models require strong multidisciplinary collaborations between different specialistic organisations… and of course the funding that comes with it.

Raissa and Jeffrey are committed to continue their exploratory conversation on models related to aging:
‘During the Helpathon we have learnt -to our relief- that many researchers struggle with the exact same problems as we do. The Helpathon helped in making contacts with researchers with a lot of expertise in studying aging, hands-on experience in brain organoids and in manufacturing 3D cellular microenvironments.’

Frank has obtained a more clear view on what is and what is not becoming possible and what is still ‘lala land’:
'While reinforcing existing and establishing new contacts with people who are pioneering animal-free pulmonary platforms, it became clear that the complexity of physiology and immunology is too extensive to tackle at this moment the complete applied research objective (...) But, on a positive note, we reconfirmed the relevance of our continuing efforts in trying to define an in vitro biomarker assay to measure correlates of in vivo protection. By using cells from a vaccinated host for such in vitro assays, we could eventually - if successful - make infectious challenges of animals redundant in the future.’

Anne-Marie is looking forward to starting new collaborations:
‘During the Helpathon, my research question remained unchanged, but I now know a lot more about which 3D liver models are available and who has the expertise.(...) Beginning next year we will start to exploit the putative collaborations and hopefully do some pilot experiments. If we get this model in place this will surely help us in unraveling the mysterious dormant parasite biology.‘

The Helpathon team is pleased with the results:
‘I lived in a tunnel’ a participant declared during the check out. The team points out that, as scientific researchers, we create our own world: our field of research, the people in our team, other colleagues in the same research field etc. We some times forget that there are other worlds out there as well. Worlds related to our research field or project. But these other worlds exist, and they can provide new insights from a different reality. The cross-border principle, looking beyond the boundaries of your own world, can make a special and surprising contribution to the research. During this Helpathon many participants travelled over the bridge instead of driving through their tunnel…’

Please watch this movie to get an impression of the event:

HELPATHON#3

IDENTIFYING LOW HANGING FRUIT FOR ANIMAL FREE LIVER RESEARCH AND DESIGNING AN ANIMAL FREE INNOVATION COURSE

We went hybrid in HELPATHON #3, June 19-20th. Due to all active and inspiring participants and to Daniela and Saskia - who were brave enough to ask for help. Watch the video to get a glimpse of what happened in the ONLINE HELPATHON HOUSE:

'The TPI HELPATHON was a great inspiration for me. As a scientist that actively makes use of animal experimentation to answer research questions in liver physiology, it is usually difficult to engage with people that seem to be opposed to using animals in research at all.' Saskia van Mil

If you want to know more please read Saskia's letter. And we will keep you posted on how Daniela’s course on animal free innovation materialises.

'At this point I felt that we were the group of teachers giving the course. We all had taken on board the responsibility to make it happen.' Daniela Salvatori

HELPATHON#2

CHALLENGED TO JUMP TO RESEARCH ON HUMAN TISSUE

"We have learned that perhaps less pre-clinical research is needed to complete our test of potential medicines in humans / patients. It is now clearer for us which steps need to be taken and that the use of animals is not always necessary. Thanks to this TPI Helpathon we have gained new insights about existing in vitro / cell culture systems that we can use to test our therapies. These models can give results that may be more relevant to humans than results obtained from laboratory animals."

Helpathon#2 was initiated by Karin Eizema from the Dutch Heart Foundation. She challenged three researchers with a animal testing practice to participate in a Helpathon.

Developing and validating a vaccine against atherosclerosis in humans takes a long time; can Bram and Ilze accelerate this by using animal-free alternatives? — Bram Slütter en Ilze Bot are both university teachers and researchers at the Leiden Academic Centre for Drug Research, in the division of Biotherapeutics.

Based on insights gained during the Helpathon, Bram and Ilze felt encouraged to pursue their pioneering work on human tissues. After the Helpathon Karin, Bram and Ilze created a research plan to further develop this animal free research practice with other Helpathon participants. Together, they successfully applied for a grant to develop a new, animal-free model to test medicines and vaccines against atherosclerosis, using human tissues recovered from surgery. The new research model is now being optimized. They expect to be able to test active substances in the near future.

When mice drink extracellular vesicles of cow milk this has benign effect on joint infections, can Fons capitalize on his finding for human health without further testing on animals? - Fons van de Loo is program leader at the Advanced biological therapy and biomarker research Department of Experimental Rheumatology, Radboud Academic Medical Center.

During HELPATHON#2 we also tackled this second question. The best way to capitalize seemed to start a business around his finding. The big question remained the health claim for humans. The latest update is that Fons can carry out more research to show these extracellular vesicles positively influence the human intestine. With the help of Karin and other people involved in the Helpathon Fons successfully applied for a grant to further progress his work in an animal free way. His project is called: Restore with extracelullar vesicles the function of the intestinal tract. due to the corona break out, his research is on hold now. Meanwhile Fons has become an active animal free innovation ambassador and is challenging his peers to look again at their research practice involving animals.

HELPATHON#1

REPURPOSING AS AN OPPORTUNITY TO DEVELOP AND VALIDATE A NEW ANIMAL FREE MODEL

Can we study the mechanisms of and develop a treatment for dynamic burn wound deepening and treatment without animal testing?

The concept of Helpathon was first tried out at the Dutch Burns Foundation who strongly promotes animal free innovation in the winter of 2018. The Helpathon was organized around the following question: 'can we study the mechanisms of and develop a treatment for dynamic burn wound deepening and treatment without animal testing?'

Researcher Paul Krijnen and funder Carine van Schie were all set to use mice to investigate the efficacy of a particular substance in inhibiting burn wound deepening and thereby positively affecting burn wound healing. During the Helpathon Paul was challenged to develop an organ on chip model to mimic human burn wounds and to use results from this model instead of animals to test the drug and to correlate these results to a clinical study in humans. Once validated, the organ on chip model can be used for the future testing of new substances. The latest update is that Paul has started the project of the development and validation of his burn wound organ on chip model in Amsterdam UMC.

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